Part:BBa_K404316
EGFP
eGPP | |
---|---|
BioBrick Nr. | BBa_K404316 |
RFC standard | RFC 25 |
Requirement | pSB1C3 |
Submitted by | [http://2010.igem.org/Team:Freiburg_Bioware FreiGEM 2010] |
EGFP was improved by the iGEM team Freiburg_Bioware 2010 by converting it into RFC 25 standard.
GFP
is a fluorescent protein,
consisting of 238 aa (=26,9 kDa), which emits green light after being
exposed
to blue light. Osamu Shimomura described the first GFP protein derived
from
Aequorea Victoria in 1962. Its excitation spectrum has two peaks at 396
and 475
nm [Heim et al., 1995]. Due to the second excitation peak at 475 nm,
GFP
protein is very photo stable [Chalfie et al., 1994].
eGFP (=enhanced GFP) is a potential derivate of GFP, which finds broad
usage in
mammalian cells. Compared to the wild-type protein, eGFP is a codon
optimized
GFP and is achieves up to 10fold higher expression rate and up to 30
fold
higher bioluminescence
Usage and Biology
eGFP is used as a marker gene for detection of transfected cells, e.g. tumor cells.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 641
References
Cormack, B.P., Valdivia, R.H. & Falkow, S., 1996. FACS-optimized mutants of the green fluorescent protein (GFP). Gene, 173(1 Spec No), pp.33-38. Available at: http://www.ncbi.nlm.nih.gov/pubmed/8707053.
Heim, R., Cubitt, A.B. & Tsien, R.Y., 1995. Improved green fluorescence. Nature, 373(6516), pp.663-664. Available at: http://www.nature.com/nature/journal/v373/n6516/abs/373663b0.html.
Chalfie M, Tu Y, Euskirchen G, Ward W, Prasher D 1994. "Green fluorescent protein as a marker for gene expression". Science 263 (5148): 802–5
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